Contact an Account ManagerSimultaneous determination of isosorbide dinitrate and diazepam in pharmaceutical preparations by HPLC with electrochemical detection
Summary
Type: HPLC
Application #: 104560
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: Fig. 3: amperometric, SMDE, -1.15V (pulse mode, basis potential -0.4V, pulse amplitude -0.75V), current: 10µA (0 to 10min), 1µA (11 to 15min).
Fig. 4: UV, 254nm, recorder full scale 1mV (0 to 10min),
Conditions: eluent: 50% methanol - 6% ethyl acetate - acetate buffer pH 6
flow rate 1.5ml/min
Authors: Kral K
Source: Z Anal Chem 316 (1983) 497 - 500
Details
Legend: Fig. 3. Chromatogram of 1. oxygen, 2. isosorbide dinitrate, 3. nitroglycerol
(int. std.), 4. diazepam, injection volume 20µl.
Fig. 4. Chromatogram of 1. and 2. unknown, 3. isosorbide dinitrate, 4.